Što je PCR test na koronavirus?
A PCR test is short for polymerase chain reaction. This test is used to detect the coronavirus. The test detects the genetic material of the virus in a patient's blood. The test is ordered if doctors suspect a patient has the coronavirus.
To accurately diagnose the coronavirus, scientists at the National Institutes of Health use a specific set of diagnostics called the polymerase chain reaction (PCR). PCR is a technique that uses enzymes naturally present in the body to amplify a viral sample. This amplification process allows doctors to detect subtle changes between infected and uninfected people by examining how the virus-amplified DNA fragments respond to specific different reagents. "PCR amplifications used to diagnose human disease are unique because they use specific primers and end fragments and involve knowledge of the patient's internal anatomy," an NIH press release reads. These unique PCR diagnostics for coronavirus use a reagent called the polymerase chain reaction (PCR) to amplify DNA.
Koje su prednosti PCR-a u odnosu na druge testove?
PCR, short for "polymerase chain reaction," is a powerful technique used to amplify (or make more) DNA present in tiny samples. The PCR portion of modern diagnostic tests is a standard part of antifungal drug diagnosis. It is widely used in coronavirus diagnostic laboratories because PCR can amplify the genetic material (or genetic code) of a virus or bacteria.
Zašto koristiti PCR umjesto drugih metoda dijagnosticiranja virusa?
-Zato što pre-digitalne tehnike kao što su tekuća kromatografija (LC) i plinska kromatografija (GC) mogu sačuvati mnoge karakteristike uzorka, uključujući promjene u DNK koja se može izdvojiti. Time se čuvaju podaci za daljnju upotrebu u istraživanju ili medicinskom istraživanju.
- Još jedna razlika. LC i GC su radno intenzivni i mogu biti skupi. PCR se, s druge strane, može izvesti u dnevnoj sobi ili bolničkoj kafeteriji. Također, prilično je jednostavan za rukovanje, što ga čini najboljim izborom za izolaciju pozitivnih uzoraka za daljnje proučavanje u kliničkom okruženju.
- Jedna vrsta PCR-a koja je nedavno dobila veliku pozornost je reverzna-amplifikacija (RPA). To je proširenje LC-a koje pomaže ubrzati brzinu i učinkovitost dijagnosticiranja nekih bakterijskih i virusnih infekcija.
